Application Of Tissue Culture In Agriculture And Horticulture Pdf

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Pocket K No. 14: Tissue Culture Technology

Plant tissue culture as an important tool for the continuous production of active compounds including secondary metabolites and engineered molecules. Novel methods gene editing, abiotic stress can improve the technique. Humans have a long history of reliance on plants for a supply of food, shelter and, most importantly, medicine. Current-day pharmaceuticals are typically based on plant-derived metabolites, with new products being discovered constantly. Nevertheless, the consistent and uniform supply of plant pharmaceuticals has often been compromised. One alternative for the production of important plant active compounds is in vitro plant tissue culture, as it assures independence from geographical conditions by eliminating the need to rely on wild plants. Plant transformation also allows the further use of plants for the production of engineered compounds, such as vaccines and multiple pharmaceuticals.

Trends in the use of tissue culture, applications and future aspects

Tissue culture as a plant production system for horticultural crops pp Cite as. The use of tissue culture with vegetable crops has an extensive history dating back to the classic studies of White 52 and Caplin and Steward 8. As a result of the efforts of many investigators in this area, techniques for micropropagation of most major species of vegetables have been developed. Most of these techniques involve the use of vegetative meristematic tissue; however, methods have also been developed in many crops for regeneration of plants from callus and from protoplasts. Due to the sheer number of plant species that are categorized as vegetables, a comprehensive literature review is not possible within the scope of this article. In addition, techniques for micropropagation of many of the major vegetable crops have recently been reviewed 1, 14, Thus, this article will concentrate on describing situations where production of vegetable plants through tissue culture represents an economically viable prospect for the vegetable industry.

role of tissue culture in horticulture

Plant tissue culture is applied in the area of plant physiological and biochemical research to study the cell cycle, metabolism in cells, nutritional, morphogenetical and developmental studies in plants. Micropropagation allows the production of large numbers of plants from small pieces of the stock plant in relatively short periods of time. The in vitro cultures are now being used as tools for the study of various basic problems in experimental biology and agriculture. The two main applications of plant tissue culture in horticultural production are in propagation and sanitation. Plant Tissue Culture PTC : Definition : Plant tissue culture is a technique with which plant cells, tissues or organs are grown on artificial nutrient medium, either static or liquid, under aseptic and controlled conditions.

The first and, to date, the most extensive practical application of tissue culture techniques to horticultural crops involves the multiplication of ornarmental plant species. The early work by Morel 4 with orchids, the development of the Murashige and Skoog medium 5 , and the efforts of Murashige 6, 7 to enhance the practical side of tissue culture science stimulated the development and widespread use of tissue culture as a means of micropropagating ornamentals.

This is a technique by which new plants can be raised by the use of plant parts or cells. In , over ornamentals were introduced to the commercial market worldwide by tissue culture techniques. Acta Horticulturae, , 39— Flower harvesting, nursery maintenance, hybrid seed production and tissue culture, propagation of fruits and flowers, and food processing are highly remunerative employment options for women in rural areas. It is one of the most widely used techniques for rapid asexual in vitro propagation.

Plant tissue culture has developed widely incorporated into biotechnology, the agricultural systems being a key factor to support many pharmaceutical and industrial outcomes.

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3 Response
  1. Maria G.

    horticulture and agriculture. Applications of micropropagation. Plant tissue culture is used widely in plant science; it also.

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